Histological –Physiological Study of Thyroid Gland in White Male Rats Processing with Aluminum Chloride and Treated with Oil of Nigella sativa

Abstract

The study included two groups,  it has been designed as two experiments , in the first experiment,24 rats have divided into four subgroups  , the first subgroup received the distal water which considering the control group and the  other three subgroups have given a different aluminum chloride concentrations (80, 160, 240 mg/kg/day) for 45 days . In the second experiment 24 rats divided into four subgroups  ,the first one received a 1 ml/kg concentration of  Nigella Sativa oil, which considered as a negative control group while the rest of the subgroups received the same previous concentrations of aluminum chloride in addition to 1ml/ kg concentrations of Nigella sativa oil .

The study showed a significant reduction(p<0.05) in the thyroid gland hormones level ( T3)   in the (160 mg/kg) concentration and T4level at (240,160 mg/kg) with a significant  elevation(p<0.05) in TSH in the same concentration when compared with positive control. The treatment with Nigella sativa oil enhance the levels of T3 in the 240 mg/kg.

For the histological changes of the studied organs  , these changes represented by a degeneration of epithelial cells that surrounded the  thyroid gland follicle  , infiltration of inflammatory cells in the lumen of the follicle, then when compared with groups treated by oil of Nigella sativa,   there is a little improvement  in some of the tissues which represented by a vacaules in thyroid follicles which it refers to the activity of the gland regeneration of epithelial cells that surrounding the follicle , there's appositive effects of oil of Nigella sativ on the level of T3 and the histology of thyroid gland changes which caused by the toxicity of 240 mg/kg  of aluminum chloride .