Detection of blaSHV, blaTEM and bla CTX-M among ‎Urinary Tract Infection Escherichia coli isolates

Abstract

One hundred fifty urine samples were collected from urinary tract infection patients. We obtained 40 isolates of Escherichia coli among these clinical samples. A proportion of UTI in females 93 (62%) is higher than that in males 57 (38%). All locally isolates are multi-resistant, the highest rate of resistance was seen with Amoxicillin/clavulanic acid, and Cloxacillin. The results showed high percentage 14(35%) of class C among locally isolates , while only 4(10%) are able to produce Class A ESβL , on the other hand  (7/40) and (8/40) were ESβL neither Class A nor Class C Present and ESβL absent respectively , while among the 40 isolates only 7 isolates were Non-Typable. Three types of  β-lactamases include ESβLs genes (bla_SHV, bla_TEM, bla_CTX-M) by the use of uniplex PCR assay. The bla_SHV was detected in 1 isolates (2.5%) of the UPEC isolates, while for the bla_TEM gene the result showed that in 27 detected isolates (67.5%), there is 23 isolates (57.5%) which indicates the prevalence of  bla_CTX-M gene. The results showed the Co-existence  of bla_TEM, bla_CTX-M in 30% of isolates, while one isolate  bla_SHV, bla_TEM, bla_CTX-M  in their genome component